Mayo Health has a highly standardized sign program to maintain consistency across the system's multiple locations. Distinct color combinations are used to represent the different locations, as demonstrated by this sign in rural Glenwood City, Wisconsin, but the graphic format and the copy elements remain uniform to maintain a consistent identity.

The main identification signs are typically internally illuminated and constructed from extrusion, using routed out and pushed through white Plexiglas copy. The secondary signs are unique in that the signposts are extruded and manufactured specifically for Mayo Health.